Researchers have developed a way to grow human heart tissue that can serve as a model for the upper chambers of the heart, known as the Atria. Human-induced pluripotent stem cells (hiPCS) derived from the tissue hit, express genes, and respond to drugs in the same way as the real human Atrium. The model described in the journal on November 8th Stem Cell Reports, may be useful in assessing mechanisms of the disease and drugs in the atrial fibrillation – the most common arrhythmia.
Contrary to conventional 2D culture, stem cell cardiomyocytes were cultured so that they formed in a heart that resembles a heart rate-like 3D heart rate. Specifically, the cells demonstrated expression, contraction, contraction and relaxation kinetics, electrophysiological properties and pharmacological reactions to atrial selective drugs of the insect-free gene. According to authors, the planned cardiac tissue could act as a model for human atrium, both in mechanical studies of etheric fibrillation and in preclinical drug screening.
"This is the first time that human atrial heart disease is born in vitro from a largely unrestricted hiPSC source," says Marta Lemme, University Medical Center Hamburg-Eppendorf. "This could be useful for both academic laboratories and the pharmaceutical industry, as we need to develop an in vitro model for atrial fibrillation to test potential new drugs and the first step is to get cells that resemble human atrial fibrillation," Lemme says.
Lemme and senior study planner at the Thomas Eschenhage University Medical Center Hamburg-Eppendorf strive to achieve this goal by generating insect-like cardiomyocytes from hiPSC using vitamin A metabolite called all-trans retinoic acid. This technique involves the genetic re-conversion of human embryonic blood or skin cells into an embryonic stem cell state and then treating these immature cells with all trans-retinoic acid to convert them into peripheral cardiomyocytes.
"But the novelty of this study is the integration of hiPSC differentiation into atrial color cardioms in the 3D environment," Lemme says. "In fact, it was shown that the 3D environment favors the differentiation of the etheric phenotype compared to the conventional 2D culture. The specific value of our study is the direct comparison of 3D-constructed cardiac tissue to native human insect tissue obtained from patients and at the functional level."
More than 33 million people suffer from atrial fibrillation worldwide and the prevalence is increasing. Coordinated High Frequency Contractions Atria increases the risk of stroke, stroke, and heart failure. Unfortunately, current treatments, such as antiarrhythmics, limit the efficacy and may cause side effects. In addition, the development of new drugs has been hampered by the fact that it is difficult to isolate and maintain human brain cardiomyocytes or cardiomyocytes. Animal models have a limited prediction because they are not exactly physiological of human cardiomyocytes.
"These anesthetics are an excellent opportunity to model atrial fibrillation and test drugs," Lemme says. "However, we can still improve to improve the similarity of human aetiology, and we will have to test different ways to test the rhythm disturbances, atrial fibrillation electrical remodeling research and new potent drugs."
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